Olympus Fluoview FV1000
The FV1000 confocal enables imaging of thick specimens as well as fixed cells stained with standard fluorophores. Mounted on an inverted microscope, the motorized stage can be used for image stitching of large samples and imaging at multiple positions. Accompanying Imaris Software is available for 3D image reconstruction. In addition the FV1000 is fitted with a Becker and Hickl FLIM system for fluorescence lifetime imaging.
- Imaging fixed cells and tissue sections labelled with 3 or 4 fluorophores. For investigating cellular architecture, localisation or co-localisation.
- 3D Imaging of thicker samples such as organoids, embryos etc.
- Imaging larger structures such as organ slices using image stitching.
- Imaging multiple points on a slide.
- FLIM imaging for accurate FRET interactions or assessing local fluorophore environment.
- FluoView Spectral FV1000 Laser Scanning Microscope based on a IX81 motorized inverted microscope
- 6 laser lines
- Freely selectable ROI for laser scanning, bleaching and photoactivation
- Scan speeds of up to 4 frames/second with 512 x 512 pixels
- xy, xz, xyz, xyzt, lambda, line scan and spot scan modes
- DIC optics
- FLIM system (Becker and Hickl) for lifetime imaging
FV1000 Laser Lines
|Laser||Excitation Lines||Suitable Dyes|
|Solid State||405nm||Alexa 405, DAPI, Hoechst|
|488nm||Alexa 488, FITC, GFP, Cy2|
|515nm||Alexa 514, YFP|
|Solid State||559nm||Alexa 546, TRITC, Cy3, DiI, propidium iodide, Texas red , mCherry|
|Solid State||635nm||Alexa 633, Alexa 647, Cy5|
- Cox, G. Biological confocal microscopy. Materials Today 5: 34-41 (2002).
- Fluorescence lifetime imaging--techniques and applications. Becker W. J Microsc. 247(2):119-36 (2012)
- Protein localization in living cells and tissues using FRET and FLIM. Chen Y, Mills JD, Periasamy A. Differentiation. 71(9-10):528-41 (2003).
Selected publications from the FV1000:
- Single molecule fluorescence in situ hybridisation for quantitating post-transcriptional regulation in Drosophila brains. Yang L, Titlow J, Ennis D, Smith C, Mitchell J, Young FL, Waddell S, Ish-Horowicz D, Davis I. Methods. 126:166-176. (2017).
- Neural circuitry coordinating male copulation. Pavlou HJ, Lin AC, Neville MC, Nojima T, Diao F, Chen BE, White BH, Goodwin SF. Elife 5. pii: e20713. doi: 10.7554/eLife.20713. (2016).
- Lhx1 functions together with Otx2, Foxa2, and Ldb1 to govern anterior mesendoderm, node, and midline development. Costello I, Nowotschin S, Sun X, Mould AW, Hadjantonakis AK, Bikoff EK, Robertson EJ. Genes Dev. 29(20):2108-22. (2015).
- Cardiac lymphatics are heterogeneous in origin and respond to injury. Klotz L, Norman S, Vieira JM, Masters M, Rohling M, Dubé KN, Bollini S, Matsuzaki F, Carr CA, Riley PR. Nature. 522(7554):62-7. (2015).
- Phosphorylation of iRhom2 at the plasma membrane controls mammalian TACE-dependent inflammatory and growth factor signalling. Grieve AG, Xu H, Künzel U, Bambrough P, Sieber B, Freeman M. Elife. 6. pii: e23968. (2017).
- Nuclear phosphorylated Dicer processes double-stranded RNA in response to DNA damage. Burger K, Schlackow M, Potts M, Hester S, Mohammed S, Gullerova M. J Cell Biol. 216(8):2373-2389. (2017).