Olympus SoRa spinning disc

Room 214.00.33

The Olympus SoRa spinning disc confocal is an inverted microscope for fast 3D super resolution imaging and prolonged cell viability in time-lapse experiments. In addition the system is fitted with z-peizo, ZDC focus, 405nm FRAP module and environmental chamber for super-resolution live cell imaging.

Applications:

  • Fast, gentle imaging of live samples, possible to a resolution of ~120nm.
  • Environmental chamber for time-lapse imaging at 37oC with 5% CO2.
  • 405nm FRAP photomanipulation module

General specifications

  • Olympus IX-83 inverted frame
  • Yokogawa SoRa super-resolution spinning disc module
  • Choice of cameras for either super-resolution imaging or maximised sensitivity
  • Incubator system for live-cell imaging and temperature control
  • 4 laser lines
  • Freely selectable ROI for laser scanning, bleaching and photoactivation with 405nm laser line
  • DIC optics
  • Peizo stage insert
  • ZDC focus

Olympus SoRa Laser Lines

Laser Excitation Lines Suitable Dyes
Solid State 405nm Alexa 405, DAPI, Hoechst
Solid State 488nm Alexa 488, FITC, GFP, Cy2
Solid State 561nm Alexa 546, TRITC, Cy3, DiI, propidium iodide, Texas red , mCherry
Solid State 633nm Alexa 633, Alexa 647, Cy5

 

Objectives Available

 

Selected reading:

 

Olympus SoRa

Olympus SoRa

Training videos

Spinning Disc Theory