Olympus SoRa spinning disc
Room 214.00.09
The Olympus SoRa spinning disc confocal is an inverted microscope for fast 3D super resolution imaging and prolonged cell viability in time-lapse experiments. In addition the system is fitted with z-peizo, ZDC focus, 405nm FRAP module and environmental chamber for super-resolution live cell imaging.
Applications:
- Fast, gentle imaging of live samples, possible to a resolution of ~120nm.
- Environmental chamber for time-lapse imaging at 37oC with 5% CO2.
- 405nm FRAP photomanipulation module
General specifications
- Olympus IX-83 inverted frame
- Yokogawa SoRa super-resolution spinning disc module
- Choice of cameras for either super-resolution imaging or maximised sensitivity
- Incubator system for live-cell imaging and temperature control
- 4 laser lines
- Freely selectable ROI for laser scanning, bleaching and photoactivation with 405nm laser line
- DIC optics
- Peizo stage insert
- ZDC focus
Olympus SoRa Laser Lines
| Laser | Excitation Lines | Suitable Dyes |
|---|---|---|
| Solid State | 405nm | Alexa 405, DAPI, Hoechst |
| Solid State | 488nm | Alexa 488, FITC, GFP, Cy2 |
| Solid State | 561nm | Alexa 546, TRITC, Cy3, DiI, propidium iodide, Texas red , mCherry |
| Solid State | 633nm | Alexa 633, Alexa 647, Cy5 |
Objectives Available
Selected reading:
Olympus SoRa
Training videos
Help Sheets
Spinning Disc Theory
