Olympus Fluoview FV1200

Room 214.00.25

The FV1200 confocal is an inverted IX83 microscope equipped with sensitive GASP detectors, a second 'SIM' scan-head for DNA damage laser experiments and an environmental chamber for live cell work. The motorized stage can be used for image stitching of large samples and time-lapse imaging at multiple positions. Accompanying Imaris Software is available for 3D image reconstruction.

Applications:

  • Imaging fixed tissue culture cells tissue sections labelled with 3 or 4 fluorophores for cellular architecture, localisation or co-localisation studies.
  • Imaging of thicker samples such as organoids, embryos etc.
  • ‘SIM' scan-head for simultaneous imaging and laser ablation (DNA damaging).
  • Environmental chamber for time-lapse imaging at 37oC with 5% CO2.
  • Programmable stage for imaging larger structures such as organ slices, or multiple points on a slide.

General Specifications

  • FluoView Spectral FV1200 Laser Scanning Microscope based on a IX83 motorized inverted microscope
  • CellVivo incubator system for live-cell imaging and temperature control
  • 6 laser lines
  • 3 standard PMT and 2 high sensitive GaAsP detectors.
  • Freely selectable ROI for laser scanning, bleaching and photoactivation
  • Scan speeds of up to 4 frames/second with 512 x 512 pixels
  • xy, xz, xyz, xyzt, lambda, line scan and spot scan modes

FV1200 Laser Lines

Laser Excitation Lines Suitable Dyes
Solid State 405nm Alexa 405, DAPI, Hoechst
Argon 458nm CFP
488nm Alexa 488, FITC, GFP, Cy2
515nm Alexa 514, YFP
Solid State 559nm Alexa 546, TRITC, Cy3, DiI, propidium iodide, Texas red , mCherry
Solid State 635nm Alexa 633, Alexa 647, Cy5

 

Objectives Available

Selected reading:

Selected publications from the FV1200:

  • HPF1/C4orf27 Is a PARP-1-Interacting Protein that Regulates PARP-1 ADP-Ribosylation Activity. Gibbs-Seymour I, Fontana P, Rack JG, Ahel I. Mol Cell. 62(3):432-42. (2016).
  • Structural insights into the function of ZRANB3 in replication stress response. Sebesta M, Cooper CDO, Ariza A, Carnie CJ, Ahel D. Nat Commun. 8:15847. (2017).
  • Acute exposure to apolipoprotein A1 inhibits macrophage chemotaxis in vitro and monocyte recruitment in vivo. Iqbal AJ, Barrett TJ, Taylor L, McNeill E, Manmadhan A, Recio C, Carmineri A, Brodermann MH, White GE, Cooper D, DiDonato JA, Zamanian-Daryoush M, Hazen SL, Channon KM, Greaves DR, Fisher EA. Elife. 5. pii: e15190. (2016).