zeiss airyscan

Zeiss 880 Ariyscan

Room 214.00.09

The Zeiss 880 confocal is an inverted microscope equipped with Airyscan ‘super-resolution detector’. In addition the system is fitted with z-peizo, definite focus, fast-Airyscan module and an environmental chamber for super-resolution live cell imaging. The motorized stage can be used for image stitching of large samples and time-lapse imaging at multiple positions.

Applications:

Imaging fixed tissue culture cells tissue sections labelled with 3 or 4 fluorophores for cellular architecture, localisation or co-localisation studies at resolution beyond that achievable with standard confocal microscopes.
Fast imaging in XY and Z dimensions
Environmental chamber for time-lapse imaging at 37oC with 5% CO2 (possible at super-resolution).
Programmable stage for imaging larger structures such as organ slices, or multiple points on a slide.

General specifications

Zeiss 880 Laser Scanning Microscope fitted with Airyscan detector
Incubator system for live-cell imaging and temperature control
6 laser lines
Freely selectable ROI for laser scanning, bleaching and photoactivation
xy, xz, xyz, xyzt, lambda, line scan and spot scan modes
DIC optics
Peizo stage insert
Definite focus

Zeiss 880 Laser Lines

Laser	Excitation Lines	Suitable Dyes
Solid State	405nm	Alexa 405, DAPI, Hoechst
Argon	458nm	CFP
	488nm	Alexa 488, FITC, GFP, Cy2
	515nm	Alexa 514, YFP
Solid State	561nm	Alexa 546, TRITC, Cy3, DiI, propidium iodide, Texas red , mCherry
Solid State	633nm	Alexa 633, Alexa 647, Cy5

Objectives Available

Selected reading:

Selected publications from the Zeiss 880 Airyscan:

An Autonomous Oscillation Times and Executes Centriole Biogenesis. Aydogan G, Steinacker T, Mofatteh M, Wilmott Z, Zhou F, Gartenmann L, Wainman A, Saurya S, Novak Z, Wong S-S, Goriely A, Boemo M, Raff J. Cell 181 (7):1566-1581. (2020).
Nucleoplasmic Reticulum Formation in Human Endometrial Cells is Steroid Hormone Responsive and Recruits Nascent Components. Pytowski L, Drozdz MM, Jiang H, Hernandez Z, Kumar K, Knott E, Vaux DJ. Int. J. Mol. Sci. 20(23), 5839. (2019).
RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage. Cristini A, Groh M, Kristiansen M, Gromak N Cell Reports 23, 1891–1905 (2018).
A homeostatic clock sets daughter centriole size in flies. Aydogan MG, Wainman A, Saurya S, Steinacker TL, Caballe A, Novak ZA, Baumbach J, Muschalik N, Raff JW. J Cell Biol. 217(4):1233-1248. (2018).
Structural Basis for Mitotic Centrosome Assembly in Flies. Feng Z, Caballe A, Wainman A, Johnson S, Haensele AFM, Cottee MA, Conduit PT, Lea SM, Raff JW. Cell. 169(6):1078-1089. (2017).
An X-ray shielded irradiation assay reveals EMT transcription factors control pluripotent adult stem cell migration in vivo in planarians. Abnave P, Aboukhatwa E, Kosaka N, Thompson J, Hill MA, Aboobaker AA. Development. pii: dev.154971. (2017).