TEM

Room 214.00.21

The FEI Tecnai 12 Transmission Electron Microscope (TEM) allows both 2D and 3D imaging of specimen ultrastructure at up to 300,000x magnification. The easy to use control console, together with the intuitive user-interface and computer-assisted alignment features, means you will be up and running on this microscope in no time! The microscope is fitted with a Bio-TWIN lens system that facilitates high contrast imaging of biological samples. Images are acquired digitally using a bottom-mounted high resolution CCD camera, although negative film and dark room processing are also available. A specialised specimen holder, together with SerialEM software, enables automated tilt series acquisition for 3D electron tomography.

Specifications:

  • Accelerating voltage: up to 120 kV
  • Lanthanum hexaboride (LaB6) electron source
  • Resolution: 0.49 nm (point)
  • Magnification: 20x to 300,000x
  • Single tilt specimen holder for standard imaging
  • Fischione 2040 dual axis tomography holder
  • Gatan Digital Micrograph and SerialEM image acquisition platforms
  • 4 Megapixel Gatan Ultrascan™ 1000 CCD camera
  • Plate camera with film

Examples of use:

  • Examination of negatively stained particulate samples (eg: viruses, bacteria, protein and liposomes)
  • Charcterisation of cellular ultrastructure (eg: mitochondria, centrosomes etc)
  • Subcellular localisation of proteins using immunogold labelling
  • Electron tomography, produces high resolution 3D reconstructions of organelles and particulate samples
monocyte

Blood-brain barrier in mouse brain tissue (A Douglas, DPAG)

 

C. elegans

Single celled algae (E Johnson/M Eason-Hubbard, Earth Sciences)

 

C. elegans

Negatively stained SAS-6 protein (E Johnson/M Cottee, Dunn School)

 

C. elegans

Mouse embryonic fibroblasts (E Johnson/A Moncada Pazos, Dunn School)

 

If you would like to find out more about this microscope or would like to discuss TEM imaging options for your research, please contact Errin Johnson.